Bonita Springs is a city in Lee County, Florida, United States. The population was 53,644 at the 2020 census. It is part of the Cape Coral-Fort Myers, Florida Metropolitan Statistical Area, on the state's southwest coast.

The city is located on both Estero Bay and the Gulf of Mexico. The area referred to as Bonita Beach is located on a road-accessible barrier island (Little Hickory Island) that lies between the Gulf of Mexico and Estero Bay. The Imperial River flows through the downtown district of the city and empties out into Estero Bay.